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Cortisol Urine Test

Urinary Free Cortisol
Sample Report
Specimen Type: Urine


Cortisol Urine Test

This test uses a 24 hour urine collection to measure a person’s Cortisol levels.  Cortisol is a hormone produced by the adrenal glands.  Proper Cortisol levels are important for helping the body to maintain a number of functions such as blood pressure, glucose levels, immune response, and stress management.  Cortisol testing is usually ordered to aid in the diagnosis of conditions related to the adrenal and pituitary glands. A Cortisol Urine test is typically ordered to aid in the diagnosis of Cushing’s Syndrome or hypercortisolism, a condition caused by elevated Cortisol production.

Cushing’s Syndrome is typically indicated by:

  • Pads of fat on the chest and upper back
  • Hair Loss
  • Irregular sleep patterns
  • Diabetes
  • High blood pressure
  • Tendency to bruise easily
  • Fatigue
  • High blood sugar levels     
  • Emotional changes including irritability and depression

Cortisol levels tend to fluctuate throughout the day.  Urine testing can provide a more accurate measurement of overall Cortisol level as it evaluates the total Cortisol production for an entire day unlike a blood test which will only show the Cortisol level at the time the blood was collected.

Conditions such as pregnancy, elevated stress levels, certain medications and hormone therapy can all affect Cortisol levels.  It is important to take these into account when results are interpreted. 

Turnaround time for the Cortisol Urine test is typically 4-5 business days.

Note: Result turn around times are an estimate and are not guaranteed. Our reference lab may need additional time due to weather, holidays, confirmation/repeat testing, or equipment maintenance.


Before beginning collection for this test, the person must receive a specimen container from the lab.  Begin by urinating at 8:00 am and discarding the specimen.  Then, collect all urine for a 24 hour period.  Keep the specimen container at room temperature.  After 24 hours, return the container to the lab.



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Offer expires December 14, 2016.


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